منابع مشابه
Quantitative Heteroduplex Analysis
General DNA analysis includes detection of targets, as well as identification and quantification of specific variants. Genomic DNA exists in the form of homoduplexes, with all corresponding base pairs being complementary, A:T and C:G. We call a double-stranded DNA (dsDNA) molecule a heteroduplex when it contains any noncomplementary base pairs. Conformational and thermodynamic changes produced ...
متن کاملQuantitative heteroduplex analysis for single nucleotide polymorphism genotyping.
High-resolution melting of polymerase chain reaction (PCR) products can detect heterozygous mutations and most homozygous mutations without electrophoretic or chromatographic separations. However, some homozygous single nucleotide polymorphism (SNPs) have melting curves identical to that of the wild-type, as predicted by nearest neighbor thermodynamic models. In these cases, if DNA of a known r...
متن کاملHeteroduplex analysis of avian RNA tumor viruses.
Electron microscopic heteroduplex analysis of avian RNA tumor viruses has been undertaken by using 35S viral RNA and long, complementary DNA synthesized in vitro. In this initial study, heteroduplex molecules were formed between complementary DNA from Rous sarcoma virus [Prague B strain (Pr-B)] and RNAs from Pr-B and Rous sarcoma virus [Prague C strain (Pr-C)] and from their transformation defe...
متن کاملHeteroduplex cleavage analysis using S1 nuclease.
A variety of methods have been developed to screen for genetic mutations. Most rely on the comparative analysis of polymerase chain reaction (PCR) products. Single-strand conformational polymorphism (4), the most widely used technique, relies on sequence-dependent conformational electrophoretic mobility differences between wild-type (WT) and mutant strands; whereas, heteroduplex analysis (5,8),...
متن کاملSimplified hepatitis C virus genotyping by heteroduplex mobility analysis.
Heteroduplex mobility analysis (HMA) was used to genotype hepatitis C viruses (HCV) with PCR fragments derived from the 5' untranslated region (5'-UTR) or the NS5b region. HCV 5'-UTR fragments were amplified from 296 serum samples by use of a combined reverse transcription-PCR assay, and the genotypes of isolates were determined by sequencing. HCV genotype distributions in Australia were 39% fo...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2007
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2007.087072